A sensitive and specific RNAse protection assay (RPA) has been used to demonstrate that CYP2B2 mRNA is highly inducible in vitroby PB. This response occurs in a time and dose dependent manner. The use of RPA and Western blotting has demonstrated that this primary rat hepatocyte culture system supports the induction of CYP2B2 mRNA and protein levels by PB.Sequencing 1.4kb of the 5′ flanking region of the CYP2B2 gene identified genomic regulatory elements and highlighted the location of the phenobarbital response element (PBRE).
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